hela gfp rab7 line Search Results


99
ATCC hela gfp rab7 line
Hela Gfp Rab7 Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sino Biological rab7
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96
Santa Cruz Biotechnology anti rab7
Anti Rab7, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc rab 7
Rab 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Santa Cruz Biotechnology rab7
The autophagic flux was blocked in BC3 cells undergoing KSHV lytic cycle activation. ( A ) Analysis of K-bZIP lytic antigen, <t>RAB7</t> and LC3-I/-II expression in the presence or in the absence of Baf (used for the last 3 h at 20 nM) in BC3 cells induced to enter the lytic cycle by T/B treatment for 36 h or ( B ) in the KSHV-negative BJAB cells undergoing the same treatment. ( C ) Kinetic analysis of the expression of K-bZIP, RAB7 and LC3-II and effects of Baf on the expression of K-bZIP, RAB7 and LC3-II in BC3 cells induced to enter the lytic cycle by T/B at the indicated time points. ACTB was used as loading control and a representative experiment out of 3 is shown. The histograms represent the mean plus SD of the densitometric analysis of the ratio of specific proteins to ACTB of 3 different experiments. ( D ) BC3 cells were transfected with pEGFP-LC3 plasmid and induced to enter the lytic cycle by T/B treatment for 0, 18 and 36 h. Lysosomes were stained in red with LysoTracker while autophagosomes were indicated by GFP-LC3 puncta. Bar: 5 micron. ( E ) Gallery of BC3 cells transfected with pDest-mCherry-EGFP-LC3B plasmid and treated with T/B for 36 h or starved for 2 h. The yellow puncta indicate autophagosomes while red puncta indicate autolysosomes. A representative experiment out of 3 is shown. Bar: 5 micron.
Rab7, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
OriGene gfp rab 7
The autophagic flux was blocked in BC3 cells undergoing KSHV lytic cycle activation. ( A ) Analysis of K-bZIP lytic antigen, <t>RAB7</t> and LC3-I/-II expression in the presence or in the absence of Baf (used for the last 3 h at 20 nM) in BC3 cells induced to enter the lytic cycle by T/B treatment for 36 h or ( B ) in the KSHV-negative BJAB cells undergoing the same treatment. ( C ) Kinetic analysis of the expression of K-bZIP, RAB7 and LC3-II and effects of Baf on the expression of K-bZIP, RAB7 and LC3-II in BC3 cells induced to enter the lytic cycle by T/B at the indicated time points. ACTB was used as loading control and a representative experiment out of 3 is shown. The histograms represent the mean plus SD of the densitometric analysis of the ratio of specific proteins to ACTB of 3 different experiments. ( D ) BC3 cells were transfected with pEGFP-LC3 plasmid and induced to enter the lytic cycle by T/B treatment for 0, 18 and 36 h. Lysosomes were stained in red with LysoTracker while autophagosomes were indicated by GFP-LC3 puncta. Bar: 5 micron. ( E ) Gallery of BC3 cells transfected with pDest-mCherry-EGFP-LC3B plasmid and treated with T/B for 36 h or starved for 2 h. The yellow puncta indicate autophagosomes while red puncta indicate autolysosomes. A representative experiment out of 3 is shown. Bar: 5 micron.
Gfp Rab 7, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hela+gfp+rab7+line/RAB7+(RAB7A)+(NM_004637)+Human+Tagged+ORF+Clone/10__1111_slash_j__1574___695x__2011__00853__x-67-13-14
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95
Cell Signaling Technology Inc mouse anti rab 7
The autophagic flux was blocked in BC3 cells undergoing KSHV lytic cycle activation. ( A ) Analysis of K-bZIP lytic antigen, <t>RAB7</t> and LC3-I/-II expression in the presence or in the absence of Baf (used for the last 3 h at 20 nM) in BC3 cells induced to enter the lytic cycle by T/B treatment for 36 h or ( B ) in the KSHV-negative BJAB cells undergoing the same treatment. ( C ) Kinetic analysis of the expression of K-bZIP, RAB7 and LC3-II and effects of Baf on the expression of K-bZIP, RAB7 and LC3-II in BC3 cells induced to enter the lytic cycle by T/B at the indicated time points. ACTB was used as loading control and a representative experiment out of 3 is shown. The histograms represent the mean plus SD of the densitometric analysis of the ratio of specific proteins to ACTB of 3 different experiments. ( D ) BC3 cells were transfected with pEGFP-LC3 plasmid and induced to enter the lytic cycle by T/B treatment for 0, 18 and 36 h. Lysosomes were stained in red with LysoTracker while autophagosomes were indicated by GFP-LC3 puncta. Bar: 5 micron. ( E ) Gallery of BC3 cells transfected with pDest-mCherry-EGFP-LC3B plasmid and treated with T/B for 36 h or starved for 2 h. The yellow puncta indicate autophagosomes while red puncta indicate autolysosomes. A representative experiment out of 3 is shown. Bar: 5 micron.
Mouse Anti Rab 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Geneservice ltd dhc-1 clones
The autophagic flux was blocked in BC3 cells undergoing KSHV lytic cycle activation. ( A ) Analysis of K-bZIP lytic antigen, <t>RAB7</t> and LC3-I/-II expression in the presence or in the absence of Baf (used for the last 3 h at 20 nM) in BC3 cells induced to enter the lytic cycle by T/B treatment for 36 h or ( B ) in the KSHV-negative BJAB cells undergoing the same treatment. ( C ) Kinetic analysis of the expression of K-bZIP, RAB7 and LC3-II and effects of Baf on the expression of K-bZIP, RAB7 and LC3-II in BC3 cells induced to enter the lytic cycle by T/B at the indicated time points. ACTB was used as loading control and a representative experiment out of 3 is shown. The histograms represent the mean plus SD of the densitometric analysis of the ratio of specific proteins to ACTB of 3 different experiments. ( D ) BC3 cells were transfected with pEGFP-LC3 plasmid and induced to enter the lytic cycle by T/B treatment for 0, 18 and 36 h. Lysosomes were stained in red with LysoTracker while autophagosomes were indicated by GFP-LC3 puncta. Bar: 5 micron. ( E ) Gallery of BC3 cells transfected with pDest-mCherry-EGFP-LC3B plasmid and treated with T/B for 36 h or starved for 2 h. The yellow puncta indicate autophagosomes while red puncta indicate autolysosomes. A representative experiment out of 3 is shown. Bar: 5 micron.
Dhc 1 Clones, supplied by Geneservice ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Geneservice ltd elpc-1 clones
The autophagic flux was blocked in BC3 cells undergoing KSHV lytic cycle activation. ( A ) Analysis of K-bZIP lytic antigen, <t>RAB7</t> and LC3-I/-II expression in the presence or in the absence of Baf (used for the last 3 h at 20 nM) in BC3 cells induced to enter the lytic cycle by T/B treatment for 36 h or ( B ) in the KSHV-negative BJAB cells undergoing the same treatment. ( C ) Kinetic analysis of the expression of K-bZIP, RAB7 and LC3-II and effects of Baf on the expression of K-bZIP, RAB7 and LC3-II in BC3 cells induced to enter the lytic cycle by T/B at the indicated time points. ACTB was used as loading control and a representative experiment out of 3 is shown. The histograms represent the mean plus SD of the densitometric analysis of the ratio of specific proteins to ACTB of 3 different experiments. ( D ) BC3 cells were transfected with pEGFP-LC3 plasmid and induced to enter the lytic cycle by T/B treatment for 0, 18 and 36 h. Lysosomes were stained in red with LysoTracker while autophagosomes were indicated by GFP-LC3 puncta. Bar: 5 micron. ( E ) Gallery of BC3 cells transfected with pDest-mCherry-EGFP-LC3B plasmid and treated with T/B for 36 h or starved for 2 h. The yellow puncta indicate autophagosomes while red puncta indicate autolysosomes. A representative experiment out of 3 is shown. Bar: 5 micron.
Elpc 1 Clones, supplied by Geneservice ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95
Genecopoeia rab7 rabbit mab
The autophagic flux was blocked in BC3 cells undergoing KSHV lytic cycle activation. ( A ) Analysis of K-bZIP lytic antigen, <t>RAB7</t> and LC3-I/-II expression in the presence or in the absence of Baf (used for the last 3 h at 20 nM) in BC3 cells induced to enter the lytic cycle by T/B treatment for 36 h or ( B ) in the KSHV-negative BJAB cells undergoing the same treatment. ( C ) Kinetic analysis of the expression of K-bZIP, RAB7 and LC3-II and effects of Baf on the expression of K-bZIP, RAB7 and LC3-II in BC3 cells induced to enter the lytic cycle by T/B at the indicated time points. ACTB was used as loading control and a representative experiment out of 3 is shown. The histograms represent the mean plus SD of the densitometric analysis of the ratio of specific proteins to ACTB of 3 different experiments. ( D ) BC3 cells were transfected with pEGFP-LC3 plasmid and induced to enter the lytic cycle by T/B treatment for 0, 18 and 36 h. Lysosomes were stained in red with LysoTracker while autophagosomes were indicated by GFP-LC3 puncta. Bar: 5 micron. ( E ) Gallery of BC3 cells transfected with pDest-mCherry-EGFP-LC3B plasmid and treated with T/B for 36 h or starved for 2 h. The yellow puncta indicate autophagosomes while red puncta indicate autolysosomes. A representative experiment out of 3 is shown. Bar: 5 micron.
Rab7 Rabbit Mab, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Bioss rab7 polyclonal antibody
The autophagic flux was blocked in BC3 cells undergoing KSHV lytic cycle activation. ( A ) Analysis of K-bZIP lytic antigen, <t>RAB7</t> and LC3-I/-II expression in the presence or in the absence of Baf (used for the last 3 h at 20 nM) in BC3 cells induced to enter the lytic cycle by T/B treatment for 36 h or ( B ) in the KSHV-negative BJAB cells undergoing the same treatment. ( C ) Kinetic analysis of the expression of K-bZIP, RAB7 and LC3-II and effects of Baf on the expression of K-bZIP, RAB7 and LC3-II in BC3 cells induced to enter the lytic cycle by T/B at the indicated time points. ACTB was used as loading control and a representative experiment out of 3 is shown. The histograms represent the mean plus SD of the densitometric analysis of the ratio of specific proteins to ACTB of 3 different experiments. ( D ) BC3 cells were transfected with pEGFP-LC3 plasmid and induced to enter the lytic cycle by T/B treatment for 0, 18 and 36 h. Lysosomes were stained in red with LysoTracker while autophagosomes were indicated by GFP-LC3 puncta. Bar: 5 micron. ( E ) Gallery of BC3 cells transfected with pDest-mCherry-EGFP-LC3B plasmid and treated with T/B for 36 h or starved for 2 h. The yellow puncta indicate autophagosomes while red puncta indicate autolysosomes. A representative experiment out of 3 is shown. Bar: 5 micron.
Rab7 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The autophagic flux was blocked in BC3 cells undergoing KSHV lytic cycle activation. ( A ) Analysis of K-bZIP lytic antigen, RAB7 and LC3-I/-II expression in the presence or in the absence of Baf (used for the last 3 h at 20 nM) in BC3 cells induced to enter the lytic cycle by T/B treatment for 36 h or ( B ) in the KSHV-negative BJAB cells undergoing the same treatment. ( C ) Kinetic analysis of the expression of K-bZIP, RAB7 and LC3-II and effects of Baf on the expression of K-bZIP, RAB7 and LC3-II in BC3 cells induced to enter the lytic cycle by T/B at the indicated time points. ACTB was used as loading control and a representative experiment out of 3 is shown. The histograms represent the mean plus SD of the densitometric analysis of the ratio of specific proteins to ACTB of 3 different experiments. ( D ) BC3 cells were transfected with pEGFP-LC3 plasmid and induced to enter the lytic cycle by T/B treatment for 0, 18 and 36 h. Lysosomes were stained in red with LysoTracker while autophagosomes were indicated by GFP-LC3 puncta. Bar: 5 micron. ( E ) Gallery of BC3 cells transfected with pDest-mCherry-EGFP-LC3B plasmid and treated with T/B for 36 h or starved for 2 h. The yellow puncta indicate autophagosomes while red puncta indicate autolysosomes. A representative experiment out of 3 is shown. Bar: 5 micron.

Journal: Autophagy

Article Title: The activation of KSHV lytic cycle blocks autophagy in PEL cells

doi: 10.1080/15548627.2015.1091911

Figure Lengend Snippet: The autophagic flux was blocked in BC3 cells undergoing KSHV lytic cycle activation. ( A ) Analysis of K-bZIP lytic antigen, RAB7 and LC3-I/-II expression in the presence or in the absence of Baf (used for the last 3 h at 20 nM) in BC3 cells induced to enter the lytic cycle by T/B treatment for 36 h or ( B ) in the KSHV-negative BJAB cells undergoing the same treatment. ( C ) Kinetic analysis of the expression of K-bZIP, RAB7 and LC3-II and effects of Baf on the expression of K-bZIP, RAB7 and LC3-II in BC3 cells induced to enter the lytic cycle by T/B at the indicated time points. ACTB was used as loading control and a representative experiment out of 3 is shown. The histograms represent the mean plus SD of the densitometric analysis of the ratio of specific proteins to ACTB of 3 different experiments. ( D ) BC3 cells were transfected with pEGFP-LC3 plasmid and induced to enter the lytic cycle by T/B treatment for 0, 18 and 36 h. Lysosomes were stained in red with LysoTracker while autophagosomes were indicated by GFP-LC3 puncta. Bar: 5 micron. ( E ) Gallery of BC3 cells transfected with pDest-mCherry-EGFP-LC3B plasmid and treated with T/B for 36 h or starved for 2 h. The yellow puncta indicate autophagosomes while red puncta indicate autolysosomes. A representative experiment out of 3 is shown. Bar: 5 micron.

Article Snippet: The knockdown of BECN1 (Santa Cruz Biotechnology, sc-29797) or RAB7 (Santa Cruz Biotechnology, sc-29460) was performed in PEL cell lines using specific small interfering RNA duplex.

Techniques: Activation Assay, Expressing, Transfection, Plasmid Preparation, Staining

The autophagic flux was blocked in TRExBCBL1-Rta cells undergoing KSHV lytic cycle activation by doxycycline treatment. ( A ) Evaluation of the autophagic flux based on LC3-II accumulation in the presence or in the absence of Baf (used for the last 3 h at 20 nM) in TRExBCBL1-Rta cells induced to enter the lytic cycle by treatment with doxycycline for 48 h. TRExBCBL1-vector cells were used as control. ( B ) The expression of KSHV lytic antigen K8.1 and RAB7 was analyzed by western blot. TUBA1A was used as loading control and a representative experiment out of 3 is shown. The histograms represent the mean plus SD of the densitometric analysis of the ratio of LC3-II, K8.1 or RAB7 to TUBA1A of 3 different experiments. ( C ) Percentage of cells expressing K8.1 (red) lytic antigen analyzed by immunofluorescence. Nuclei were stained by DAPI (blue).

Journal: Autophagy

Article Title: The activation of KSHV lytic cycle blocks autophagy in PEL cells

doi: 10.1080/15548627.2015.1091911

Figure Lengend Snippet: The autophagic flux was blocked in TRExBCBL1-Rta cells undergoing KSHV lytic cycle activation by doxycycline treatment. ( A ) Evaluation of the autophagic flux based on LC3-II accumulation in the presence or in the absence of Baf (used for the last 3 h at 20 nM) in TRExBCBL1-Rta cells induced to enter the lytic cycle by treatment with doxycycline for 48 h. TRExBCBL1-vector cells were used as control. ( B ) The expression of KSHV lytic antigen K8.1 and RAB7 was analyzed by western blot. TUBA1A was used as loading control and a representative experiment out of 3 is shown. The histograms represent the mean plus SD of the densitometric analysis of the ratio of LC3-II, K8.1 or RAB7 to TUBA1A of 3 different experiments. ( C ) Percentage of cells expressing K8.1 (red) lytic antigen analyzed by immunofluorescence. Nuclei were stained by DAPI (blue).

Article Snippet: The knockdown of BECN1 (Santa Cruz Biotechnology, sc-29797) or RAB7 (Santa Cruz Biotechnology, sc-29460) was performed in PEL cell lines using specific small interfering RNA duplex.

Techniques: Activation Assay, Plasmid Preparation, Expressing, Western Blot, Immunofluorescence, Staining

RAB7 knockdown leads to an autophagic block in PEL cells. BCBL1 cells were knocked down for RAB7 or scramble (SC) treated and ( A ) RAB7 and ( B ) LC3-II expression level was evaluated in the presence or in the absence of Baf. TUBA1A was used as loading control and a representative experiment out of 3 is shown. The histograms represent the mean plus SD of the densitometric analysis of the ratio of LC3-II to TUBA1A of 3 different experiments.

Journal: Autophagy

Article Title: The activation of KSHV lytic cycle blocks autophagy in PEL cells

doi: 10.1080/15548627.2015.1091911

Figure Lengend Snippet: RAB7 knockdown leads to an autophagic block in PEL cells. BCBL1 cells were knocked down for RAB7 or scramble (SC) treated and ( A ) RAB7 and ( B ) LC3-II expression level was evaluated in the presence or in the absence of Baf. TUBA1A was used as loading control and a representative experiment out of 3 is shown. The histograms represent the mean plus SD of the densitometric analysis of the ratio of LC3-II to TUBA1A of 3 different experiments.

Article Snippet: The knockdown of BECN1 (Santa Cruz Biotechnology, sc-29797) or RAB7 (Santa Cruz Biotechnology, sc-29460) was performed in PEL cell lines using specific small interfering RNA duplex.

Techniques: Blocking Assay, Expressing